Study on Chlamydia trachomatis Antigen Detection by an EIA Kit Using a Monoclonal Antibody
نویسندگان
چکیده
منابع مشابه
Prevalence of Chlamydia trachomatis Antigen and Antibody in Infertile Women in Ahwaz
Chlamydia trachomatis is an obligate intracellular bacterium which causes a wide variety of human infections such as ocular, urogenital and respiratory infections. Genital infections of women, espe-cially when repeated, give rise to many complications such as ectopic pregnancy, miscarriage and infertility. Since chlamydial infections are usually asymptomatic, they progress unnoticed and produc...
متن کاملChlamydia trachomatis antigen detection in pregnancy and its verification by antibody blocking assay.
PURPOSE To detect the prevalence of genital infection caused by Chlamydia trachomatis in pregnant women and also to confirm the positive results using blocking antibody assay. METHODS Endocervical specimens were collected from 200 symptomatic and asymptomatic pregnant women attending the ANC OPD at M P Shah Medical College, Jamnagar. The samples were tested for presence of Chlamydia trachomat...
متن کاملEvaluation of a genus reactive monoclonal antibody in rapid identification of Chlamydia trachomatis by direct immunofluorescence.
This study compared the direct visualisation of elementary bodies in urogenital smears by the direct immunofluorescence test with the isolation of Chlamydia trachomatis in McCoy cell cultures treated with cytochalasin B. C trachomatis was isolated from 41 (16.4%) of 250 unselected specimens in McCoy cell cultures treated with cytochalasin B. Of the 41 culture positive specimens 37 (90%) were di...
متن کاملDetection of Chlamydia trachomatis endocervical infections by ligase chain reaction versus ACCESS Chlamydia antigen assay.
Ligase chain reaction (LCR) was compared with ACCESS immunoassay for detection of chlamydial infections in females. Despite efforts to improve ACCESS performance by evaluation of specimens that were in the test performance "grey zone," LCR remained more sensitive and was less expensive to perform. ACCESS had a sensitivity of 83.9%, a specificity of 99.7%, a positive predictive value of 96.3%, a...
متن کاملPurification of Chlamydia trachomatis strains in mixed infection by monoclonal antibody neutralization.
A Chlamydia trachomatis D* serovariant strain was found to be mixed with an F serovar strain in a clinical specimen. By using a monoclonal antibody which neutralizes serovar F infectivity in hamster kidney cells, the D* variant strain was enriched until it could be cloned by limiting dilution. This newly described neutralization enrichment procedure can be used to purify a C. trachomatis serova...
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ژورنال
عنوان ژورنال: Journal of the Japanese Association for Infectious Diseases
سال: 1991
ISSN: 1884-569X,0387-5911
DOI: 10.11150/kansenshogakuzasshi1970.65.457