Studies on the Cucumber Mosaic Virus in Tobacco Plant

Solubility Studies on Purified Tobacco Mosaic Virus

Different samples of purified tobacco mosaic virus show a relatively wide variation in solubility in ammonium sulfate solution. This variation and the type of solubility curve obtained in the presence of varying amounts of solid phase show that the purified virus whether isolated by mild treatment with ammonium sulfate or by ultracentrifugation is not a homogeneous chemical substance but contai...

Cucumber mosaic virus and its 2b RNA silencing suppressor modify plant-aphid interactions in tobacco

The cucumber mosaic virus (CMV) 2b protein not only inhibits anti-viral RNA silencing but also quenches transcriptional responses of plant genes to jasmonic acid, a key signalling molecule in defence against insects. This suggested that it might affect interactions between infected plants and aphids, insects that transmit CMV. We found that infection of tobacco with a 2b gene deletion mutant (C...

Infection of tobacco mesophyll protoplasts by cucumber mosaic virus.

Protoplasts isolated from tobacco leaves were inoculated with cucumber mosaic virus (CMV) using a method slightly modified from that developed for inoculating tobacco mosaic virus. Multiplication of CMV was demonstrated by staining the protoplasm with fluorescent CMV-antibody, by the electron microscopy of ultrathin sections, and by assaying the infectivity in protoplasts. Seventy to 90% of pro...

Study of resistance to cucumber mosaic virus watermelon mosaic virus and zucchini yellow mosaic virus in melon cultivars

Chemical Studies on the Virus of Tobacco Mosaic

A method for the isolation, from mosaic-diseased Turkish tobacco plants, of a crystalline protein possessing the properties of tobacco mosaic virus has been described (1, 2). The method consists essentially in precipitation of the globulin fraction from a dilute disodium phosphate extract of frozen, macerated, infected plants with ammonium sulfate, and the removal of most of the remaining color...