STUDIES ON RAT LIVER CHOLINE OXIDASE: AN ASSAY METHOD
نویسندگان
چکیده
منابع مشابه
The distribution of choline oxidase activity in rat liver.
Rat liver was fractionated essentially as described by Hogeboom, Schneider, and Pallade (2). The liver was homogenized in 0.88 M sucrose (1 gm. of liver to 10 ml.) in the cold and centrifuged in 25 ml. Lustroid tubes in a 32” angle centrifuge (Custom Scientific Instruments, Inc.) in a cold room kept at 5”. The homogenate was separated into nuclear, mitochondrial, and supernatant fractions. The ...
متن کاملOxidation of choline-like substances by rat liver preparations; inhibitors of choline oxidase.
The work of Moyer and du Vigneaud (1) has demonstrated that, of a considerable number of compounds related to choline, choline itself, betaine, its sulfur analogue dimethylthetin, and the dimethylethyl analogue of choline, i.e. dimethylethyl-P-hydroxyethylammonium chloride, are the only ones which will permit rat growth on a methionine-free homocystinecontaining diet. Subsequent work by Dubnoff...
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In studying the oxidation of xanthine manometrically by using liver homogenate systems (l), certain irregularities in the method have become evident to the authors. Occasionally an oxygen consumption has been observed after 2 to 3 hours of incubation which is greatly in excess of the theoretical requirement for the complete oxidation of the xanthine substrate. In certain experiments the measure...
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An h.p.l.c. method was developed for the assay of coproporphyrinogen oxidase activity in rat liver. The protoporphyrinogen IX formed is completely oxidized to protoporphyrin IX for separation and quantification by reversed-phase chromatography with mesoporphyrin as the internal standard. The Km of coproporphrinogen oxidase is 1.01 +/- 0.23 microM. The activities are 4.07 +/- 0.40 nmol of protop...
متن کاملStudies on the stability of the choline oxidase.
The choline oxidase was first described by Bernheim and Bernheim (1). Later it was shown (2) that choline at pH 6.7 was oxidized to betaine aldehyde which was then only slowly oxidized further, whereas at pH 7.8 the oxidation proceeded rapidly to betaine. This indicated that betaine aldehyde was oxidized by another enzyme system, and Klein and Handler (3) were able to show that its oxidation re...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1951
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)55908-1