Studies on ram acrosin. Fluorimetric titratiion of operational molarity with 4-methylumbelliferyl p-guanidinobenzoate
نویسندگان
چکیده
منابع مشابه
Thermodynamic analysis reveals structural rearrangement during the acylation step in human trypsin 4 on 4-methylumbelliferyl 4-guanidinobenzoate substrate analogue.
Human trypsin 4 is an unconventional serine protease that possesses an arginine at position 193 in place of the highly conserved glycine. Although this single amino acid substitution does not affect steady-state activity on small synthetic substrates, it has dramatic effects on zymogen activation, interaction with canonical inhibitors, and substrate specificity toward macromolecular substrates....
متن کاملIdentification of Salmonellae with the 4-methylumbelliferyl caprilate fluorescence test.
We have tested 750 Salmonella strains and 130 strains of other species of the family Enterbacteriaceae with the 4-methylumbelliferyl caprilate reagent (MUCAP) test. The MUCAP test is a fluorescence test for rapid identification of Salmonella strains. The non-Salmonella strains were strains sent for identification as suspected Salmonella strains and thus have phenotypes similar to those of Salmo...
متن کاملPrevention of fertilization by exposure of hamster eggs to soluble acrosin.
Incubation of hamster eggs with trypsin or chymotrypsin, but not with a series of glycosidases or lipases, prevents capacitated spermatozoa from binding to the zona pellucida so that fertilization is prevented (Hartmann & Gwatkin, 1971 ; Gwatkin, Williams & Andersen, 1973). Inactivation of the binding sites for spermatozoa on the zona pellucida by a trypsin-like protease released by discharge o...
متن کاملStudies on the acute pathologic effects of 4-(p-dimethyl
aminostyryl)quinoline (4M20) in mice (3, 10) indicated that the compound had certain radiomimetic effects. It was also noted that lens cataracts occurred in animals surviving the acute effects (5). A striking nonradio mimetic feature of the acute lesions was the marked hepa totoxic effect of the drug. This was manifested by fatty liver and necrosis of liver cells during the 1st week, followed b...
متن کاملP-159: Effect of Silymarin on Viability, Motility and Mitochondrial Memberane Potential of Ram Spermatozoa Treated with Aluminum Chloride
Background: Aluminum, as an environmental pollutant, can exert oxidative stress on spermatogenesis and sperm morphology. Silymarin, an effective substance extracted from Silybum marianum, is a potent antioxidant which inhibits oxidative stress. Due to the damaging effects of aluminum on the male reproductive system as well as sperm and the role of silymarin, this study was performed to investig...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1975
ISSN: 0264-6021
DOI: 10.1042/bj1490147