Studies on osteoclasts. Identification and characterization of osteoclasts in bone marrow cell culture.
نویسندگان
چکیده
منابع مشابه
Membrane currents of murine osteoclasts generated from bone marrow/stromal cell co-culture.
Whole cell clamp recordings were conducted to examine the general behavior of ion channels in osteoclasts which were generated from bone marrow/stromal cell co-culture. In the resting state, inwardly and outwardly rectifying (IR and OR) currents were observed in the osteoclasts cultured for 5-18 days and the whole cell currents were classified into three types according to the conductance ratio...
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The effect of Pasteurella multocida toxin (PMT) on porcine osteoclast and osteoblast differentiation was studied using in vitro cell culture systems. When grown in the presence of Vitamin D3, isolated porcine bone marrow cells formed multinucleated cells with features characteristic of osteoclasts. Exposure of bone marrow cells to Vitamin D3 and PMT during growth resulted in formation of increa...
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Formation of the hematopoietic stem cell (HSC) niche in bone marrow (BM) is tightly associated with endochondral ossification, but little is known about the mechanisms involved. We used the oc/oc mouse, a mouse model with impaired endochondral ossification caused by a loss of osteoclast (OCL) activity, to investigate the role of osteoblasts (OBLs) and OCLs in the HSC niche formation. The absenc...
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BACKGROUND Osteoporosis is a skeletal disease leading to an increased risk of bone fracture. Using a mouse osteoporosis model induced by administration of a receptor activator of nuclear factor kappa-B ligand (RANKL), salubrinal was recently reported as a potential therapeutic agent. To evaluate the role of salubrinal in cellular fates as well as migratory and adhesive functions of osteoclast/o...
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A carborundum wheel was used to prepare slices of cortical bone that demonstrate a predictable surface appearance in the scanning electron microscope. Osteoclasts were mechanically disaggregated from neonatal rabbit long bones and settled onto these slices. After 24h in culture osteoclasts were associated with areas of excavation in the bone surface. These excavated areas typically showed a wel...
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ژورنال
عنوان ژورنال: Japanese Journal of Oral Biology
سال: 1986
ISSN: 0385-0137
DOI: 10.2330/joralbiosci1965.28.472