Studies of Reduced Diphosphopyridine Nucleotide Oxidase: Effects of Isooctane Extraction on Enzymatic Catalysis
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منابع مشابه
Studies of reduced diphosphopyridine nucleotide oxidase: effects of isooctane extraction on enzymatic catalysis.
In previous reports Nason et al. (l-3) have demonstrated that the activity of preparations of reduced diphosphopyridine nucleotide cytochrome c reductase extracted with 2,2,4-trimethylpentane (isooctane) was restored by addition of oc-tocopherol or a number of other compounds. Stotz et al. (4)) Marinetti et al. (5)) and Deul et al. (6) have reported studies similar to those of Nason. In other e...
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DPNH-X was prepared as described previously (1) ; the barium salt was converted to the sodium salt before use, and solutions were stored at 20”. Concentrations of DPNH-X were calculated with an extinction coefficient of 13.5 X lo6 sq. cm. mole-’ at 290 rnM (1). Sodium salts of ADP, ATP, ITP, UTP, and crystalline disodium ATP were obtained from the Pabst Laboratories, Milwaukee, Wisconsin. Myoki...
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It has been previously shown (l-3) that the nicotinamide moiety of diphosphopyridine nucleotide can be replaced by other pyridine bases without the loss of the coenzyme function. The observation that both the 3-acetylpyridine and pyridine-3aldehyde analogues of diphosphopyridine nucleotide function as coensymes with various dehydrogenases (3) indicated that the amide group on DPN was not essent...
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Few studies on purified bacterial reduced diphosphopyridine nucleotide (DPNH) or cytochrome oxidases have been reported, although cytochrome reduction by DPNH’ in crude bacterial extracts has repeatedly been described (l-6). Dolin (7, 8) purified a flavoprotein DPNH oxidase and peroxidase from Streptococcus jaecalis, and Lenhoff and Kaplan (9) and Lenhoff et al. (10) described the characteristi...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1959
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)98179-2