STUDIES OF MEMBRANE FORMATION IN TETRAHYMENA PYRIFORMIS
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چکیده
منابع مشابه
Studies of Membrane Formation in Tetrahymena Pyriformis
A method has been devised to fractionate cells of Tetrahymena pyriformis, yielding pure or highly enriched preparations of cilia, cilia-associated soluble material, pellicles, mitochondria, microsomes, and postmicrosomal supernatant. The method prevents the destructive action of lipolytic enzymes commonly associated with this organism. Analysis of the membrane lipids of these fractions reveals ...
متن کاملSTUDIES OF MEMBRANE FORMATION IN TETRAHYMENA PYRIFORMIS II . Isolation and Lipid Analysis of Cell Fractions
A method has been devised to fractionate cells of Tetrahymena pyriformis, yielding pure or highly enriched preparations of cilia, cilia-associated soluble material, pellicles, mitochondria, microsomes, and postmicrosomal supernatant . The method prevents the destructive action of lipolytic enzymes commonly associated with this organism . Analysis of the membrane lipids of these fractions reveal...
متن کاملCiliary Membrane Differentiations in Tetrahymena Pyriformis
We have examined thin sections and replicas of freeze-fractured cilia of Tetrahymena pyriformis. The ciliary necklace located at the base of all freeze-fractured oral and somatic cilia has been studied in thin sections. Since electron-dense linkers have been found to connect both microtubule doublets and triplets to the ciliary membrane at the level of the necklace, the linkers and the associat...
متن کاملRespiratory Enzyme Studies in Tetrahymena pyriformis
Meyerhof, 0. (1945). J. biol. Chem. 157, 105. Meyerhof, 0. & Kaplan, A. (1951). Arch. Biochem. Biophy8. 38, 282. Meyerhof, 0. & Oesper, P. (1947). J. biol. Chem. 170, 1. Meyerhof, 0. & Wilson, J. R. (1949). J. biol. Chem. 180, 575. Nelson, N. (1944). J. biol. Chem. 153, 373. Nilsson, R. & Alm, F. (1941). Biochem. Z. 308, 262. Prankerd, T. A. J. & Altman, K. I. (1954). Biochem. J. 58, 622. Roe, ...
متن کاملSTUDIES OF MEMBRANE FORMATION IN TETRAHYMENA PYRIFORMIS III. Lipid Incorporation into Various Cellular Membranes of Logarithmic Phase Cultures
When 114C-palmitic acid is used to pulse label logarithmic cultures of Tetrahymena pyriformis, radioactivity appears in lipids of the various membrane types at vastly differing rates . The microsomes and postmicrosomal supernatant attain a high specific radioactivity within 1 min, while the membranes enveloping the cilia require several hours to reach the microsomal level. A similar pattern is ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1971
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.49.3.722