Structure-Sweetness Relationship in Thaumatin: Importance of Lysine Residues
نویسندگان
چکیده
منابع مشابه
Structure-sweetness relationship in thaumatin: importance of lysine residues.
To clarify the structural basis for the sweetness of thaumatin I, lysine-modified derivatives and carboxyl-group-modified derivatives were prepared by chemical modification followed by chromatographic purification. The sweetness of derivatives was evaluated by sensory analysis. Phosphopyridoxylation of lysine residues Lys78, Lys97, Lys106, Lys137 and Lys187 markedly reduced sweetness. The inten...
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Thaumatin, an intensely sweet-tasting plant protein, elicits a sweet taste at 50 nM. Although the sweetness remains when thaumatin is heated at 80 °C for 4h under acid conditions, it rapidly declines when heating at a pH above 6.5. To clarify the structural difference at high pH, the atomic structure of a recombinant thaumatin I at pH 8.0 was determined at a resolution of 1.0Å. Comparison to th...
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ژورنال
عنوان ژورنال: Chemical Senses
سال: 2001
ISSN: 1464-3553
DOI: 10.1093/chemse/26.2.167