Structure probing of tmRNA in distinct stages of trans-translation

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Various effects of paromomycin on tmRNA-directed trans-translation.

trans-Translation is an unusual translation in which tmRNA plays a dual function as a tRNA and an mRNA to relieve the stalled translation on the ribosome. In this study, we examined the effects of an aminoglycoside antibiotic, paromomycin, on several tmRNA-related events in vitro. The results of a chemical footprinting study indicated that paromomycin molecules bind tmRNA at G332/G333 in the tR...

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SmpB as the handyman of tmRNA during trans-translation.

In the recent years, a wealth of genetic, biochemical and structural data focusing on various steps of bacterial trans-translation was reported. The early events, from stalled ribosome recognition, pre-accommodation to translocation have been recently investigated in great details. In comparison, the later events including 'elongation-termination' onto tmRNA reading frame and ribosome recycling...

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tRNA/mRNA Mimicry by tmRNA and SmpB in Trans-Translation

Since accurate translation from mRNA to protein is critical to survival, cells have developed translational quality control systems. Bacterial ribosomes stalled on truncated mRNA are rescued by a system involving tmRNA and SmpB referred to as trans-translation. Here, we review current understanding of the mechanism of trans-translation. Based on results obtained by using directed hydroxyl radic...

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ژورنال

عنوان ژورنال: RNA

سال: 2007

ISSN: 1355-8382

DOI: 10.1261/rna.451507