Structure of the recombinant antibody Fab fragment f3p4

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Structure of the recombinant antibody Fab fragment f3p4.

The structure of the antibody Fab fragment f3p4, which was selected from a subset of the synthetic HuCAL antibody library to bind the sodium citrate symporter CitS, is described at 1.92 A resolution. Comparison with computational models revealed deviations in a few framework positions and in the binding loops. The crystals belong to space group P2(1)2(1)2 and contain four molecules in the asymm...

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Human snake bitten poisoning is a serious threat in many tropical and subtropical countries such as Iran. The best acceptable treatment of envenomated humans is antivenoms; however they have a series of economic and medical problems and need more improvements. In this study a combinatorial human immunoglobulin gene library against some of Iranian snakes venoms was constructed. Total RNA prepare...

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Characterization of a recombinant humanized anti-cocaine monoclonal antibody and its Fab fragment

Variations of post-translational modifications are important for stability and in vivo behavior of therapeutic antibodies. A recombinant humanized anti-cocaine monoclonal antibody (h2E2) was characterized for heterogeneity of N-linked glycosylation and disulfide bonds. In addition, charge heterogeneity, which is partially due to the presence or absence of C-terminal lysine on the heavy chains, ...

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construction of a human recombinant polyclonal fab fragment antibody library using peripheral blood lymphocytes of snake bitten victims

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ژورنال

عنوان ژورنال: Acta Crystallographica Section D Biological Crystallography

سال: 2008

ISSN: 0907-4449

DOI: 10.1107/s0907444908007282