Structure of PAP-IgM FcK fusion protein with J-chain expressed in transgenic plant
نویسندگان
چکیده
Abstract Transgenic plants expressing immunoglobulin (Ig) M Fc-fused Prostate acid phosphatase (PAP) antigenic proteins (PAP-IgM FcK) and J-chain were generated by Agrobacterium-mediated transformation. The Fc region was tagged with the ER retention motif (KDEL) to make PAP-IgM FcK. Two transgenic crossed together generate F 1 both FcK × J-chain). PCR RT-PCR analyses confirmed transgene insertion mRNA transcription of in leaf tissue plant. Western blot expression protein. Size exclusion (SEC)-high performance liquid chromatography (HPLC) Bio-transmission electron microscope (TEM) performed show size shape PAP- IgM fusion proteins. These results suggest that can be produced plant system crossing.
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ژورنال
عنوان ژورنال: The eurobiotech journal
سال: 2023
ISSN: ['2564-615X']
DOI: https://doi.org/10.2478/ebtj-2023-0006