Structural studies on the Escherichia coli O101 lipopolysaccharide found in association with F41 and K99 fimbriae

Structural studies on the Escherichia coli O101 lipopolysaccharide found in association with F41 and K99 fimbriae.

In this study it was shown that the O101 lipopolysaccharide isolated from Escherichia coli B41 did not contain an O-specific polysaccharide and that its sugar moiety is probably restricted to the core oligosaccharide. It is characterized by the presence of galactose, glucose, N-acetylglucosamine, heptose and 3-deoxy-D-manno-2-octulosonic acid and the fatty acid composition is typical of an Ente...

Confirmed nucleotide sequence of fanF of Escherichia coli K99 fimbriae.

Nucleotide sequence of the F41 fimbriae subunit gene in Escherichia coli B41.

A clone encoding the F41 f imbnal sub-unit gene from E coli B41, was isolated from an B1BL3 library using an oligonucleotide based on the published N-termlnal amino acid sequence (1). The DNA sequence of an t I66bp fragment revealed an open reading frame encoding a protein of 277 amino acids including a 22 amino acid leader sequence (dash underlined). This corresponds to a mature protein of 290...

Passive immunity in cattle against enterotoxigenic Escherichia coli: serologic evaluation of a bacterin containing K99 and F41 fimbriae in colostrum of vaccinated females and calf serum

A bacterin from enterotoxigenic Escherichia coli (ETEC), containing fimbriae K99 and F41, was produced and its capacity to induce anti-K99 and anti-F41 antibodies in colostrum of vaccinated cows and in calf serum, and the persistence of these antibodies in neonates were determined. Three experiments were performed on two commercial farms. In all experiments animals were allotted randomly to the...

The nucleotide sequence of the genes, fanE and fanF of Escherichia coli K99 fimbriae.

K99 fimbriae of enterotoxigenic Escherichia coli consist of eight different subunits. A major subunit called fimbrillin forms fimbrial structure and a minor subunit called adhesin localizes at the tip of fimbriae and recognizes host receptor ganglioside. Within this eight gene cluster, fanE and fanF have not yet been sequenced. In this study, fanE and fanF genes were sequenced by analyzing seve...