Structural Rearrangements of Sucrose Phosphorylase from Bifidobacterium adolescentis during Sucrose Conversion
نویسندگان
چکیده
منابع مشابه
Increased Cellulose Production from Sucrose by Acetobacter after Introducing the Sucrose Phosphorylase Gene.
A sucrose phosphorylase (SPase) gene derived from Leuconostoc mesenteroides was introduced into a cellulose-producing Acetobacter strain and expressed under the lac promoter. The activity of the SPase was detected in extracts of the transformed cells and cellulose production from sucrose by the cells was found to have increased, which strongly suggests that the increase was the result of the ne...
متن کاملPurification and mechanism of action of sucrose phosphorylase.
Sucrose phosphorylase (disaccharide glucosyltransferase) from Pseudomonas saccharophila has been highly purified. The enzyme appears to be homogeneous in the ultracentrifuge, on starch gel electrophoresis and on polyacrylamide gel disc electrophoresis. The molecular weight of the enzyme as determined by Sephadex chromatography is 80,000 to 100,000. The kinetics of the reaction was examined and ...
متن کاملNoteFormation of Kojibiose and Nigerose by Sucrose Phosphorylase
of sucrose and inerganic phosphate to or-D-g]ucosc-1-phosphatc (G-1-P) and D-fructose,') We have reported that thc SPase from Leueonostoe niesenteroides had a rather broad acceptor specificity and transferred the glucosyl moicty of G-1-P to various sugars and sugar alcohols, These findings werc studjed using high performance liquid chromatography (HPLC) under the fol]owing conditions: column, T...
متن کاملNoteFormation of Kojibiose and Nigerose by Sucrose Phosphorylase
of sucrose and inerganic phosphate to or-D-g]ucosc-1-phosphatc (G-1-P) and D-fructose,') We have reported that thc SPase from Leueonostoe niesenteroides had a rather broad acceptor specificity and transferred the glucosyl moicty of G-1-P to various sugars and sugar alcohols, These findings werc studjed using high performance liquid chromatography (HPLC) under the fol]owing conditions: column, T...
متن کاملBiocatalytic Process for Production of a-Glucosylglycerol Using Sucrose Phosphorylase
Glycosylglycerols are powerful osmolytes, produced by various plants, algae and bacteria in adaptation to salt stress and drought. Among them, glucosylglycerol (2-O-a-D-glucopyranosyl-sn-glycerol; GG) has attracted special attention for its promising application as a moisturizing agent in cosmetics. A biocatalytic process for the synthesis of GG as industrial fine chemical is described in which...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2006
ISSN: 0021-9258
DOI: 10.1074/jbc.m605611200