Structural Evidence of a Passive Base-flipping Mechanism for β-Glucosyltransferase
نویسندگان
چکیده
منابع مشابه
The coupling of tight DNA binding and base flipping: identification of a conserved structural motif in base flipping enzymes.
Val(121) is positioned immediately above the extrahelical cytosine in HhaI DNA C(5)-cytosine methyltransferase, and replacement with alanine dramatically interferes with base flipping and catalysis. DNA binding and k(cat) are decreased 10(5)-fold for the Val(121) --> Ala mutant that has a normal circular dichroism spectrum and AdoMet affinity. The magnitude of this loss of function is comparabl...
متن کاملOn base flipping
Although change and adaptation are key to life, nature is reluctant to abandon old inventions. Even today when the hypothetical RNA world of our ancestors has been supplanted by the richer possibilities offered by proteins, we still find key processes catalyzed by RNA. Perhaps the oldest discovery was the unique power of base pairing to enable replication. This must have been key to the develop...
متن کاملDNA base flipping by a base pair-mimic nucleoside
On the basis of non-covalent bond interactions in nucleic acids, we synthesized the deoxyadenosine derivatives tethering a phenyl group (X) and a naphthyl group (Z) by an amide linker, which mimic a Watson-Crick base pair. Circular dichroism spectra indicated that the duplexes containing X and Z formed a similar conformation regardless of the opposite nucleotide species (A, G, C, T and an abasi...
متن کاملA rapid reaction analysis of uracil DNA glycosylase indicates an active mechanism of base flipping
Uracil DNA glycosylase (UNG) is the primary enzyme for the removal of uracil from the genome of many organisms. A key question is how the enzyme is able to scan large quantities of DNA in search of aberrant uracil residues. Central to this is the mechanism by which it flips the target nucleotide out of the DNA helix and into the enzyme-active site. Both active and passive mechanisms have been p...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2004
ISSN: 0021-9258
DOI: 10.1074/jbc.m404394200