Structural characterization of an exopolysaccharide produced by <i>Lactobacillus plantarum</i> Ts

Abstract An exopolysaccharide (EPS) was isolated from Lactobacillus plantarum Ts and purified by size exclusion chromatography train. L. showed the highest production (9.4 ± 0.8 g/l) of EPS. Furthermore, cultured in medium with volume 5 L EPS extracted ethanol precipitation. By HPLS analysis demonstrated, that has two fractions - polymer olygomer. The molecular mass both is respectively 8.3x104 Da for greater pic 5.0x102 Da. Fourier transform infrared spectroscopy presence hydroxyl carboxyl groups glycosidic linkages. TLC indicated a heteropolymer composed fructose, sucrose as monomeric constituent units. strain pathogenic E. coli 3398, St. aureus 745, B. subtilis 6633, S. typhimurium 3591, monocytogenes 863 aerogenes 3691 were tested their growth utilizing sole carbon source its possible use prebiotic. exhibited supplied compared source, whereas strains did not grow EPS-supplied medium. In vitro evaluations that, like other reported polysaccharides, this displayed significant prebiotics properties.