Structural Analysis of Aliphatic versus Aromatic Substrate Specificity in a Copper Amine Oxidase fromHansenula polymorpha
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Kinetic and structural analysis of substrate specificity in two copper amine oxidases from Hansenula polymorpha.
The structural underpinnings of enzyme substrate specificity are investigated in a pair of copper amine oxidases (CAOs) from Hansenula polymorpha (HPAO-1 and HPAO-2). The X-ray crystal structure (to 2.0 A resolution) and steady state kinetic data of the second copper amine oxidase (HPAO-2) are presented for comparison to those of HPAO-1. Despite 34% sequence identity and superimposable active s...
متن کاملStructural studies of copper amine oxidase
Introduction Copper amine oxidases are ubiquitous metalloenzymes. Their function in prokaryotes and lower eukaryotes is to utilise amines as a source for carbon and nitrogen. In higher eukaryotes, their roles are less well understood, but have been linked with cell signalling, growth and development, and cell death. Despite this apparent diversity, their fundamental role is to catalyse the oxid...
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In their studies of the oxidation of adrenalin and other amines by amine oxidase preparations, Blaschko, Richter, and Schlossmann (1) found that l-ephedrine, triethylamine, triisoamylamine, and dl-1-hydroxy-2-hydrindeneamine are not only not oxidized, but inhibit the action of the enzyme upon isoamylamine. The conclusion was drawn that this inhibition was of the competitive type, because some d...
متن کاملSubstrate specificity of amine oxidases.
DuBois, K. P., Albaum, H. G. & Potter, V. R. (1943). J. biol. Chem. 147, 699. Green, H. N. (1943). Lancet, 2, 147. Green, H. N. & Stoner, H. B. (1944). Brit. J. exp. Path. 25, 150. Kal¢kar, H. M. (1943). J. biol. Chem. 148, 127. Kolthoff, I. M. (1932). Saure-Ba8en Indicatoren. Berlin: Springer. Krebs, H. A. & Henseleit, K. (1932). Ho.ppe-Seyl. Z. 210, 33.' Lyubimova, M. N. & Pevsner, D. (1941)....
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The process by which molecular oxygen is activated to enable it to function as an electron acceptor in biology is poorly understood. The quinoprotein copper-containing amine oxidase (CuAO) catalyses the conversion of primary amines into aldehydes. As well as copper, the enzyme contains an organic cofactor, 2,4,5-trihydroxyphenylalanine quinone (TPQ). Following the formation of aldehyde, the enz...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2013
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi3016845