Stripe assay to examine axonal guidance and cell migration
نویسندگان
چکیده
منابع مشابه
Cell-to-substratum adhesion and guidance of axonal elongation.
The behavior of axonal growth cones on surfaces with patterned variations in substratum was observed. Cells from sensory ganglia of 8-day-old chicken embryos were cultured on plastic petri dishes, plastic tissue culture dishes, and polyornithine-coated tissue culture dishes, all of which contained gridlike patterns of palladium (Pd) deposition. The results indicated that growth cones elongated ...
متن کاملCollective guidance of collective cell migration.
Some cells migrate and find their way as solitary entities. However, during development of multicellular animals and possibly during tumor dissemination, cells often move as groups, associated tightly or loosely. Recent advances in live imaging have aided examination of such 'multicellular cell biology'. Here, I propose a model for how a group of cells can process and react to guidance informat...
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Primordial germ cells (PGCs), the progenitors of the gametes, migrate from the position where they are specified towards the region where the gonad develops. To reach their target, the PGCs obtain directional cues from cells positioned along their migration path. One such cue, the chemokine SDF-1, has recently been found to be critical for proper PGC migration in zebrafish and in mice. In Droso...
متن کاملMicrofluidic control of axonal guidance
The precision of axonal pathfinding and the accurate formation of functional neural circuitry are crucial for an organism during development as well as during adult central and peripheral nerve regeneration. While chemical cues are believed to be primarily responsible for axonal pathfinding, we hypothesize that forces due to localized fluid flow may directly affect neuronal guidance during earl...
متن کاملMAP1B Is Required for Netrin 1 Signaling in Neuronal Migration and Axonal Guidance
BACKGROUND The signaling cascades governing neuronal migration and axonal guidance link extracellular signals to cytoskeletal components. MAP1B is a neuron-specific microtubule-associated protein implicated in the crosstalk between microtubules and actin filaments. RESULTS Here we show that Netrin 1 regulates, both in vivo and in vitro, mode I MAP1B phosphorylation, which controls MAP1B activ...
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ژورنال
عنوان ژورنال: Nature Protocols
سال: 2007
ISSN: 1754-2189,1750-2799
DOI: 10.1038/nprot.2007.157