Storage of skin biopsies at -70 degrees C for future fibroblast culture.

Measurementof ApolipoproteinB in Plasma from HyperlipidemicSubjectsTreated with Cholestyramineor Placebo: DifferentialEffectsof Storage at -70 #{176}C

Using radial immunodiffusion, we measured apolipoprotein B (apoB) concentrations in fresh plasma from 216 hyperlipidemic male subjects and in aliquots of the same plasma stored at -70 #{176}C for 22 months. At the time of sampling, 112 were being treated with cholestyramine, the rest with a placebo. ApoB concentrations in the stored samples were significantly and positively correlated with apoB...

Fibroblast Viability after Storage at 20 °C in Milk, Hank's Balanced Salt Solution and Coconut Water.

The objective of this study was to evaluate the effectiveness of various storage media at 20 °C in maintaining the viability of human periodontal ligament fibroblasts (HPLF) over time. HPLF were maintained at 20 °C in skim milk (SM), whole milk (WM), freshly prepared Hank's balanced salt solution (HBSS), Save-A-Tooth(r), natural coconut water (NCW), coconut water industrialized (ICW) and tap wa...

Storage of Various Cell Lines at - 70 ° C or - 80 ° C in Multi - Well Plates While Attached to the Substratum

layer to support the sample, we frequently measure proton currents traveling towards the dish floor, suggesting that this provides a more physiological environment in which ions are free to move from all surfaces of the ovariole unimpeded (Figure 3). The recording chamber provides a physiological environment and specimen manipulation and orientation possibilities that are well suited to extrace...

Long term maintenance of Theileria annulata strains by freezing at -70'C

Experimental model for fibroblast culture

The use of cell culture methods in Plastic Surgery opened a new horizon in the research of cellular mechanisms of proliferation and biosynthesis functions. Several types of cells have been investigated in the cutaneous compartment. Keratinocytes and fibroblasts have been studied aiming the possibility of developing biomaterial for skin substitution. The present study describes the standardizati...