Sterilization of the biological safety cabinet

Modified laminar flow biological safety cabinet.

Tests are reported on a modified laminar flow biological safety cabinet in which the return air plenum that conducts air from the work area to the high efficiency particulate air filters is under negative pressure. Freon gas released inside the cabinet could not be detected outside by a freon gas detection method capable of detecting 10(-6) cc/s. When T3 bacteriophage was aerosolized 5 cm outsi...

A test system for the biological safety cabinet.

A simple, cheap and readily available test system for biological safety cabinets is described. It depends on the containment of an aerosol of Bacillus subtilis spores generated in a BIRD micronebulizer and the measurement of air flows with an anemometer. The system was set up to survey new equipment but equally valuable results have been obtained from tests during use. New units were often badl...

Ergonomic considerations in the development of a class II, type A/B3 biological safety cabinet.

Inactivation of Francisella tularensis Schu S4 in a Biological Safety Cabinet Using Hydrogen Peroxide Fumigation

This study evaluated the inactivation of Francisella tularensis Schu S4 on various materials (acrylic, glass, polyamide, polyethylene, polypropylene, silicone rubber, and stainless steel) using hydrogen peroxide fumigation of a Class III Biological Safety Cabinet (BSC III). A suspension of F. tularensis Schu S4 (7 x 10 CFU) was dried on seven different types of test surfaces and exposed to vapo...

Evaluation of chemical and biological consequences of soil sterilization methods

Sterilized soils are commonly used for the study of xenobiotic sorption and as an abiotic control in biodegradation experiments. They are also used for the chemical study of nitrogen and carbon cycle processes and the elimination of the biological factors. In this research the effects of different soil sterilization methods such as autoclaving, fumigating and exposure to ultraviolet and microwa...