Step-Size Is Determined by Neck Length in Myosin V†
نویسندگان
چکیده
منابع مشابه
Step-size is determined by neck length in myosin V.
The highly processive motor, myosin V, has an extremely long neck containing six calmodulin-binding IQ motifs that allows it to take multiple 36 nm steps corresponding to the pseudo-repeat of actin. To further investigate how myosin V moves processively on actin filaments, we altered the length of the neck by adding or deleting IQ motifs in myosin constructs lacking the globular tail domain. Th...
متن کاملNeck length and processivity of myosin V.
Myosin V is an unconventional myosin that transports cargo such as vesicles, melanosomes, or mRNA on actin filaments. It is a two-headed myosin with an unusually long neck that has six IQ motifs complexed with calmodulin. In vitro studies have shown that myosin V moves processively on actin, taking multiple 36-nm steps that coincide with the helical repeat of actin. This allows the molecule to ...
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Myosin V is a motor protein from the myosin superfamily, involved in various intracellular transport processes [1]. It is a processive motor [2], which means that a single protein molecule can transport cargoes along actin filaments over distances of several micrometers. It is dimeric, consisting of two identical heads, each attached to a lever arm, joined with each other through a tail domain....
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Muscle myosins transduce ATP free energy into actin displacement to power contraction. In vivo, myosin side chains are modified post-translationally under native conditions, potentially impacting function. Single myosin detection provides the 'bottom-up' myosin characterization probing basic mechanisms without ambiguities inherent to ensemble observation. Macroscopic muscle physiological experi...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2005
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi0512086