STEM-08. MODELING AND UNDERSTANDING GLIOBLASTOMA EDGE CELLS

Abstract Glioblastoma is a devastating disease with an overall median survival of 8 months from diagnosis. The majority patients die tumor relapse in close proximity the resected primary tumor. intensely researched but most studies have been performed on tissues and cultures derived bulk while it remaining edge cells that cause lethality. Investigations are rare few experimental models exist. Here we established analyzed series matched cell outer six IDH wildtype glioblastoma purpose to understand biology. Tumor samples were guided by 5-ALA fluorescence using neuro-navigation stringent procedures not contaminate cells. First fluorescent tissue. After removal all areas careful irrigation cavity sample was 1-2 cm outside border non-eloquent area. Following dissociation used sphere assays for explantation. There significant difference self-renewal across between cultures, line results acute cells, suggesting maintenance properties cultures. Invasion analysis showed reverse strengthening general functional distinction patients. To investigate molecular basis our findings whole exome sequencing (WES) combined single RNA- ATAC-sequencing (10X Multiome). Analyses ongoing WES data does support genetic causes their differences 10X Multiome indicate epigenetic regulation may underlie different