Statistical Optimization of Alkali Pretreatment to Improve Sugars Recovery from Spent Coffee Grounds and Utilization in Lactic Acid Fermentation

Biorefinery, which utilizes carbon-neutral biomass as a resource, is attracting attention significant alternative in modern society confronted with climate change. In this study, spent coffee grounds (SCGs) were used the feedstock for lactic acid fermentation. order to improve sugar conversion, alkali pretreatment was optimized by statistical method, namely response surface methodology (RSM). The optimum conditions of SCGs determined follows: 75 °C, 3% potassium hydroxide (KOH) and time 2.8 h. enzymatic hydrolysis pretreated enzyme complex loading 30-unit cellulase, 15-unit cellobiase 50-unit mannanase per g reaction 96 SCG hydrolysates carbon source Lactobacillus cultivation, conversions L. brevis ATCC 8287 parabuchneri 49374 40.1% 55.8%, respectively. Finally, maximum production estimated be 101.2 based on 1000 through optimization hydrolysis.