Statistical evaluation of confocal microscopy images
نویسندگان
چکیده
منابع مشابه
Statistical evaluation of confocal microscopy images.
BACKGROUND The coefficient of variation (CV) is defined as the standard deviation (sigma) of the fluorescent intensity of a population of beads or pixels expressed as a proportion or percentage of the mean (mu) intensity (CV = sigma/mu). The field of flow cytometry has used the CV of a population of bead intensities to determine if the flow cytometer is aligned correctly and performing properly...
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Colocalization is an important finding in many cell biological studies. This unit describes a protocol for quantitative evaluation of images with colocalization based on the calculation of a number of specialized coefficients. First, images of double-stained sections are subjected to background correction. Then, various coefficients are calculated. Meanings of the coefficients and a guide to in...
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ژورنال
عنوان ژورنال: Cytometry
سال: 2001
ISSN: 0196-4763,1097-0320
DOI: 10.1002/1097-0320(20010801)44:4<295::aid-cyto1121>3.0.co;2-c