State of knowledge: Histolocalisation in phytochemical study of medicinal plants
نویسندگان
چکیده
The renewed interest in medicinal plants has led us to examine more closely the usefulness of metabolite histolocalisation screening work before any in-depth phytochemical studies. Indeed, this method is a histochemical technique allowing characterizing plant tissues constituents; and particular metabolites therapeutic interest, without destroying or altering as much possible studied material. This aims at carring out wide highlight bioactive from our rich university heritage collection. characterisation used chemical, morphological topographical (localisation) that uses precipitation reactions using dyes, among others. To do we made thin cross-sections razor blades on fresh sections were then coloured conventional chemical stains observations MOTIC BA210 microscope equipped with MOTICAM camera. In view obtained results, technique, therefore, proves be useful analysis when applied studies such Datura stramonium, Peperomia obtusifolia, Cecropia obtusa, Orthosiphon aristatus Vitex agnus castus. results confirm presence sought metabolites, allow their precise histological localisation. will make extraction process profiTable, simpler even ecological by avoiding waste.
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ژورنال
عنوان ژورنال: Fitoterapia
سال: 2021
ISSN: ['1873-6971', '0367-326X']
DOI: https://doi.org/10.1016/j.fitote.2021.104862