منابع مشابه
A nitroxide-maleimide spin label.
was chemically bonded to poly-L-lysine and to bovine serum albumin (BSA) and showed a paramagnetic resonance spectrum that was sensitive to known structural changes in these molecules, such as those produced by changes in pH.2 This nitroxide-isocyanate is unfortunately not an ideal spin-labeling reagent since it is unstable and thus difficult to purify, and moreover the isocyanate group is rapi...
متن کاملThermal lability of membrane proteins of age separated erythrocytes as studied by electron spin resonance spin label technique.
Thermal lability of bovine erythrocyte membrane proteins was studied by electron spin resonance using maleimide spin label. The temperature of the sample during measurements could be varied for the first time between 0 and 60 degrees C with an accuracy of +/- 0.1 degrees C. Our results show that "old" erythrocyte membrane proteins are less stable against thermal denaturation then "young" cells.
متن کاملDetection of malignant hyperthermia susceptibility using a spin label technique on red blood cells.
Using spin labelled red blood cells and electron paramagnetic resonance spectroscopy (EPR), we observed that halothane 3 mmol litre-1 produced a much greater decrease in the rotational correlation time of red blood cells from individuals who were at risk for malignant hyperthermia (MH), compared with normals. Subsequently we performed blind tests on 14 individuals whose MH susceptibility status...
متن کاملHeme-Spin Label Studies of Hemoglobin
In order to investigate the protein conformation in the vicinity of the heme and the spin states of the heme-iron in dissolved hemoglobins, a nitroxide spin label was covalently attached to one of the propionic acid groups of the porphyrin ring. The optical spectra of the heme-spin labeled ferrihemoglobins were identical to those of native hemoglobins indicating that the spin labeling did not a...
متن کاملFabrication of Plasmonic Substrates Using Facile Technique of Spin-Coating for Salmonella Bacteria Detection
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ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 2010
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.50.014