Staining of Proteins on Cellulose Acetate

Estimation of serum proteins by electrophoresis on cellulose acetate.

We have evaluated results for albumin obtained by a standard procedure for cellulose acetate electrophoresis of proteins in serum. The Ponceau S-stained .albumin and globulins were eluted and the albumin was calculated by the generally accepted formula [(albumin-bound dye absorbance/absorbance of total protein-bound dye) X total serum protein concn] and by the formula (absorbance of albumin-bou...

Rapid cellulose acetate electrophoresis. I. Serum proteins.

A method of electrophoresis utilizing cellulose acetate is described wherein quantitation of serum protein fractions by densitometry is accomplished within 2 hr. of initiation of the procedure. The densitometer was found to have a linear response to amido black dye. Albumin and y-globulin demonstrated equal dye-binding characteristics. Combined with the absence of albumin trailing, this has aff...

The Staining of Lactic Dehydrogenase Isoenzymes after Electrophoretic Separation on Cellulose Acetate.

Established electrophoretic techniques for the separation of the lactate dehydrogenase (L.D.H.) isoenzymes of tissues and body fluids, although of value in clinical medicine, are too elaborate for most routine laboratories. A simple method for the separation of these isoenzymes on cellulose acetate and their histochemical staining is described. Spectrophotometric estimations of the total L.D.H....

Separation of haemoglobins on cellulose acetate.

Underestimation of monoclonal proteins by serum protein electrophoresis on cellulose acetate.

Our study of 95 serum samples from 37 patients with monoclonal gammopathy revealed distorted irregular monoclonal (M) protein bands after serum protein electrophoresis (SPE) on cellulose acetate membrane. In 71 (75%) of the 95 sera, the M-protein was underestimated and the albumin concentration overestimated. Dilution of the serum sample before SPE eliminated the abnormality of the M-protein ba...