Stable thiobarbituric acid chromophore with dimethyl sulphoxide. Application to sialic acid assay in analytical de-O-acetylation

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Stable thiobarbituric acid chromophore with dimethyl sulphoxide. Application to sialic acid assay in analytical de-O-acetylation.

With dimethyl sulphoxide instead of butanol in the thiobarbituric acid assay for sialic acid, a non-fading chromophore with lambdamax. = 549 nm was produced in a homogeneous solution, allowing dilution of the test mixture in case of high colour yield. This test adapted well to studies on alkaline de-O-acetylation. Bovine and rat submaxillary mucins, and rabbit Tamm-Horsfall urinary sialoprotein...

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The thiobarbituric acid assay of sialic acids.

Members of the sialic acid family of compounds occur in a variety of tissues and body fluids (la, 2) and in bacteria (3), incorporated in conjugated protein. The naturally occurring sinlic acids are substituted neuraminic acid derivatives (Nacetyl, N-glycolyl, N, 0-diacetylneuraminic acid). These are collectively termed “sialic acids.” The unsubstituted 9 carbon chain is called neuraminic acid ...

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Regulation of sialic acid O-acetylation in human colon mucosa.

The expression of O-acetylated sialic acids in human colonic mucins is developmentally regulated, and a reduction of O-acetylation has been found to be associated with the early stages of colorectal cancer. Despite this, however, little is known about the enzymatic process of sialic acid O-acetylation in human colonic mucosa. Recently, we have reported on a human colon sialate-7(9)-O-acetyltran...

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NeuA sialic acid O-acetylesterase activity modulates O-acetylation of capsular polysaccharide in group B Streptococcus.

Group B Streptococcus (GBS) is a common cause of neonatal sepsis and meningitis. A major GBS virulence determinant is its sialic acid (Sia)-capped capsular polysaccharide. Recently, we discovered the presence and genetic basis of capsular Sia O-acetylation in GBS. We now characterize a GBS Sia O-acetylesterase that modulates the degree of GBS surface O-acetylation. The GBS Sia O-acetylesterase ...

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O-acetylation and de-O-acetylation of sialic acids in human colorectal carcinoma.

A decrease in the level of O-acetylated sialic acids observed in colorectal carcinoma may lead to an increase in the expression of sialyl Lewis(X), a tumor-associated antigen, which is related to progression of colorectal cancer to metastasis. The underlying mechanism for this reduction is, however, not fully understood. Two enzymes are thought to be primarily responsible for the turnover of O-...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1976

ISSN: 0264-6021

DOI: 10.1042/bj1590457