Stable Long-Term Blood Formation by Stem Cells in Murine Steady-State Hematopoiesis
نویسندگان
چکیده
منابع مشابه
Long-term maintenance of hematopoiesis in irradiated mice by retrovirally transduced peripheral blood stem cells.
Mobilized peripheral blood stem cells (PBSC) are used as a source of hematopoietic stem cells for transplantation and gene therapy. It is still unclear, however, whether the PBSC are fully equivalent to normal bone marrow hematopoietic stem cells and whether they are able to provide long-term function of transgene in reconstituted mice. In the present study, mobilized PBSC from male mice were t...
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Homologous disruption of expression of signal transducer and activator of transcription 5a (STAT5a) and STAT5b (STAT5ab(-/-)) in mice results in hematopoietic stem cells (HSCs) that can engraft irradiated hosts alone but are noncompetitive against wild-type HSCs. To explore mechanisms for this phenotype, we crossed the STAT5 mutations onto an HW80 background congenic to the original C57BL/6 tha...
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background: spermatogonial stem cells (sscs), a subset of undifferentiated type a spermatogonia, are the foundation of complex process of spermatogenesis and could be propagated in vitro culture conditions for long time for germ cell transplantation and fertility preservation. objective: the aim of this study was in vitro propagation of human spermatogonial stem cells (sscs) and improvement of ...
متن کاملHEMATOPOIESIS Hematopoiesis and stem cell renewal in long-term bone marrow cultures containing catalase
Culturing mouse bone marrow in the presence of catalase dramatically alters hematopoiesis. Granulocyte output is initially increased 4to 5-fold. This increase is transient and granulocyte production declines as immature (Sca-1 /LIN ) cells accumulate. One third of these immature cells have a phenotype (Sca-1 /c-Kit ) characteristic of hematopoietic stem cells. At 2 to 3 weeks there are greater ...
متن کاملHuman hematopoiesis in murine embryos after injecting human cord blood-derived hematopoietic stem cells into murine blastocysts.
At different developmental stages, candidate human hematopoietic stem cells (HSCs) are present within the CD34+ CD38- population. By means of xenotransplantation, such CD34+CD38- cells were recently shown to engraft the hematopoietic system of fetal sheep and nonobese diabetic severe combined immunodeficient adult mice. Here it is demonstrated that, after their injection into murine blastocysts...
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ژورنال
عنوان ژورنال: STEM CELLS
سال: 2012
ISSN: 1066-5099
DOI: 10.1002/stem.1151