Stable Isotope Probing with 15 N 2 Reveals Novel Noncultivated Diazotrophs in Soil
نویسندگان
چکیده
منابع مشابه
Stable Isotope Probing Identifies Novel m-Xylene Degraders in Soil Microcosms from Contaminated and Uncontaminated Sites
The remediation of groundwater contaminated with benzene, toluene, ethylbenzene, and the xylenes (BTEX) typically involves in situ biodegradation. Although the mechanisms of aerobic BTEX biodegradation in laboratory cultures have been well studied, less is known about the microorganisms responsible in mixed culture samples or at contaminated sites. In this study, the microorganisms responsible ...
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A new approach to characterize growing microorganisms in environmental samples based on labeling microbial DNA with H(2)(18)O is described. To test if sufficient amounts of (18)O could be incorporated into DNA to use water as a labeling substrate for stable isotope probing, Escherichia coli DNA was labeled by cultivating bacteria in Luria broth with H(2)(18)O and labeled DNA was separated from ...
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A new approach to characterize growing microorganisms in environmental samples based on labeling microbial DNA with H2 O is described. To test if sufficient amounts of O could be incorporated into DNA to use water as a labeling substrate for stable isotope probing, Escherichia coli DNA was labeled by cultivating bacteria in Luria broth with H2 O and labeled DNA was separated from [O]DNA on a ce...
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11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 RNA based stable isotope probing (SIP) facilitates the detection and identification of active members of microbial populations that are involved in the assimilation of an isotopically labeled compound. N-RNA-SIP is a new method that has been discussed in recent literature but has not yet been tested. Herein, we define the limitation...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2007
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.02610-06