Stable-Isotope-Based Labeling of Styrene-Degrading Microorganisms in Biofilters
نویسندگان
چکیده
منابع مشابه
Stable Isotope-based in-situ Labelling as a Reference for the Isolation of relevant Styrene Degraders from Biofilters
The enrichment of styrene degrading microorganisms from biofilters resulted in a broad range of degrading isolates with unkown in situ relevance. For this reason an in situ labeling of styrene degrading microorganisms using deuterated styrene and phospholipid fatty acids as biomarkers prior to the isolation approach was performed. By this method the fatty acid profiles of the primary styrene de...
متن کاملLabeling of Human Serum Albumin with Stable Isotope of Bromine; an in Vitro Study
Background: Possibility to trace-label albumin with isotopes results in information concerning its synthesis, breakdown, and distribution in the intra and extra cellular spaces. The iodination of albumin is a widespread procedure used in scientific studies. Bromine not only is more reactive and less expensive than iodine, but bonds more easily with many elements. Therefore, it could be a suitab...
متن کاملStable Isotope Labeling in Proteomics
Proteomics, the analysis of the proteins expressed by a cell, tissue or organism under a specific set of conditions, has undergone a tremendous period of growth in the past few years. Proteomic studies are typically designed to analyze hundreds or thousands of proteins in a single analysis and aim to provide a global view of changes in protein expression that occur in different cellular growth ...
متن کاملIdentification of in situ 2,4-dichlorophenoxyacetic acid-degrading soil microorganisms using DNA-stable isotope probing
Stable isotope probing (SIP) was used to investigate the microorganisms responsible for degradation of the herbicide, 2,4dichlorophenoxyacetic acid (2,4-D) in soil samples. Soils were unamended or amended with either unlabeled 2,4-D or UL(ring) C-2,4D. Degradation of 2,4-D was complete after 17 days, whereas little removal (1173%) was observed in the sterile controls. Terminal restriction fragm...
متن کاملIdentification of hexahydro-1,3,5-trinitro-1,3,5-triazine-degrading microorganisms via 15N-stable isotope probing.
This study reported the application of 15N-stable isotope probing (SIP) to identify active hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX)-utilizing microorganisms in groundwater microcosms. Fifteen 16S rRNA gene sequences were derived from the 15N-DNA fraction (contributed from active microorganisms capable of using RDX as a nitrogen source) of microcosms receiving cheese whey. The 16S rRNA gene...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2001
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.67.10.4796-4804.2001