Stable glycosylated hemoglobin as measured by a microcolumn method.
نویسندگان
چکیده
منابع مشابه
Labile glycosylated hemoglobin contributes to hemoglobin A1 as measured by liquid chromatography or electrophoresis.
The utility of glycosylated hemoglobin (HbA1) measurement as an index of chronic control in diabetes can be adversely affected by interference from a labile glycosylated fraction that changes rapidly with acute changes in blood glucose concentration. I used a "high-pressure" liquid-chromatographic assay and a newly developed electrophoretic assay to quantitate the contribution of this labile fr...
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We evaluated a colorimetric assay for glycosylated hemoglobin to determine the effects of several variables --oxalic acid concentration, extraneous glucose, hemoglobin concentration, hydrolysis interval, and 5-hydroxymethylfurfural destruction--and the precision. The interference seen when the blood glucose concentration exceeds 2.0 g/L (11 mmol/L) can be eliminated by washing the erythrocytes ...
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Objective: Oxidative stress is involved in the pathophysiology of diabetes and cardiovascular complications of metabolic syndrome. The main objective of this study was to evaluate total antioxidant status by diphenylpicrylhydrazyl (DPPH)-scavenging activity in patients developed metabolic syndrome (MetS). Materials and Methods: Forty-four patients with MetS diagnosed on the basis of adult tre...
متن کاملGlycosylated hemoglobin measured by affinity chromatography: micro-sample collection and room-temperature storage.
Under proper conditions, whole blood can be stored at room temperature for as long as 21 days before measurement of glycosylated hemoglobin by affinity chromatography. Whole blood (anticoagulated with EDTA or heparin) was placed in capillary tubes, which were then sealed at both ends and stored at room temperature. Just before assay, whole blood was rinsed from the tubes and diluted 10-fold wit...
متن کاملEffects of whole blood storage on results for glycosylated hemoglobin as measured by ion-exchange chromatography, affinity chromatography, and colorimetry.
After storage of whole blood at either 4 or 20 degrees C, results for glycosylated hemoglobin by ion-exchange chromatography ("high-performance" liquid and mini-column chromatography), thiobarbituric acid colorimetry, and affinity chromatography were compared. At 4 degrees C, all methods gave acceptable results for samples stored for as long as a week. At 20 degrees C, the colorimetric and affi...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1983
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/29.9.1687