Stabilization of bromthymol blue colour in lecithin-sphingomyelin ratio determinations in amniotic fluid.
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چکیده
منابع مشابه
Simple, rapid determination of lecithin and sphingomyelin in amniotic fluid.
We describe a rapid and very sensitive modified determination of lecithin and sphingomyelin in amniotic fluid. It requires 1.5 ml of amniotic fluid and can be done in less than 40 mm. Modifications include “Instant Thin Layer Chromatography” plates (Gelman) with a punched disc system of sample application, as well as elimination of the acetone wash steps, and substitution of Rhodamine B dye for...
متن کاملNew method for determining lecithin and sphingomyelin in amniotic fluid.
We describe a new method of complete analysis for lecithin, lysolecithin, and sphingomyelin in amniotic fluid. The analysis is based on alkaline hydrolysis of the lecithins and the enzymic hydrolysis of sphingomyelin. The choline formed in each instance is enzymically phosphorylated with [gamma-32P]ATP to yield [gamma-32P]phosphorylcholine, which is isolated by anion-exchange chromatography and...
متن کاملA modified procedure for evaluation of the lecithin-sphingomyelin ratio in amniotic fluid.
We describe several modifications of the procedure originated by Gluck et al. [Amer. J. Obstet. Gynecol. 109, 440 (1971)] for evaluation of amniotic fluid lecithin/sphingomyelin (L/S) ratios. The acetone-precipitation step is eliminated, resulting in a faster extraction procedure and higher concentrations of phospholipids. For faster thin-layer chromatography, we use silica gel-impregnated shee...
متن کاملImproved procedure for lecithin/sphingomyelin ratio in amniotic fluid reduces false predictions of lung immaturity.
Many laboratories have found that their procedure for determining lecithin/sphingomyelin (L/S)ratios gives an unacceptably high proportion of false predictions of fetal lung immaturity. We investigated each step in the procedure, in an attempt to improve the clinical performance of the test and to make the method more amenable to standardization between laboratories. L/S ratios were determined ...
متن کاملRapid thin-layer chromatographic method for assessing the lecithin-sphingomyelin ratio in amniotic fluid.
Submitters: Emily H. Coch, Gerald Kessler, and John S. Meyer, Department of Pathology and Laboratory Medicine, The Jewish Hospital of St. Louis, St. Louis, Mo. 63110 Evaluators: Paul T. Russell, Department of Obstetrics and Gynecology, University of Cincinnati Medical Center, Cincinnati, Ohio 54229 Donald T. Forman, Division of Clinical Biochemistry, Evanston Hospital, Evanston, Ill. 60201 Lasz...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1976
ISSN: 0021-9746
DOI: 10.1136/jcp.29.10.908