Stability of 125 I-Labeled Staphylococcal Enterotoxins in Solid-Phase Radioimmunoassay
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چکیده
منابع مشابه
Staphylococcal enterotoxins A and B: solid-phase radioimmunoassay in food.
An immunoassay employing (125)I labeled enterotoxins A and B and polystyrene tubes coated with specific antibodies was used for detection and quantitation of enterotoxin in food. Ham salad, cheddar cheese, custard, condensed milk, and salami were studied. Enterotoxin was successfully determined in all the foods by simple extraction procedures. The assay was sensitive to 1 to 10 ng of toxin per ...
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The activity of y-glutamyltransferase (EC 2.3.2.2) is measured on the DuPont aca by an adaptation of the Szasz procedure ;-y-glutamyl-p-nitroanilide is transferred to glycylglycine, thereby releasing p-nitroaniline, which is measured at 405 nm. I have noticed low glutamyltransfer-ase activity in patients with cholestasis wherealkaline phosphate (EC 3.1.3.1) is increased, total bilirubin> 100 mg...
متن کاملRapid solid-phase radioimmunoassay for staphylococcal enterotoxin A.
A rapid solid-phase radioimmunoassay for staphylococcal enterotoxin A is described. The assay procedure requires 3 to 4 h for completion by using a competitive inhibition system in which the antibody is attached to bromacetyl cellulose particles. It is accurate to a level of 0.01 mug of enterotoxin A/ml in a variety of media such as ham, milk products, crab meat, custard, etc. No significant in...
متن کاملStaphylococcal Enterotoxins
Staphylococcus aureus (S. aureus) is a Gram positive bacterium that is carried by about one third of the general population and is responsible for common and serious diseases. These diseases include food poisoning and toxic shock syndrome, which are caused by exotoxins produced by S. aureus. Of the more than 20 Staphylococcal enterotoxins, SEA and SEB are the best characterized and are also reg...
متن کاملInvolvement of enterotoxins G and I in staphylococcal toxic shock syndrome and staphylococcal scarlet fever.
We investigated the involvement of the recently described staphylococcal enterotoxins G and I in toxic shock syndrome. We reexamined Staphylococcus aureus strains isolated from patients with menstrual and nonmenstrual toxic shock syndrome (nine cases) or staphylococcal scarlet fever (three cases). These strains were selected because they produced none of the toxins known to be involved in these...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1975
ISSN: 0003-6919
DOI: 10.1128/am.29.6.776-779.1975