منابع مشابه
Simple spot test for rapid detection of urease activity.
A spot test for the detection of urease activity was developed and evaluated with 761 strains of gram-negative bacteria. The test was compared with the conventional Christensen urea agar slants and urease test on the Vitek Enterobacteriaceae card (Vitek Systems, Inc., St. Ana, Mo.). Of the 348 strains of the Proteus-Providencia-Morganella group that were urease positive, 327 (94%) yielded posit...
متن کاملA reliable spot test for mucopolysaccharidoses.
From the Department of Ophthalmology, Biochemistry Research Laboratory (The Sir Isaac and Lady Wolfson Ophthalmic Research Laboratories), Hebrew University-Hada.ssah Medical School, Jerusalem, Israel. 1 Present address: Department of Biophysics, Weizmann InstituteofScience,Rehovot,Israel. Nonstandard abbreviations used: MPS, mucopolysaccharides (glycosaminoglycans),mucopolysaccharidoses (with r...
متن کاملSpot test for detection of sickling hemoglobin.
We describe a simple, noninstrumental procedure for detecting hemoglobin S in samples of blood on filter paper, with use of small volumes of reagents. The method depends on the differential solubilities of reduced sickling and nonsickling hemoglobin in phosphate solutions (1, 2) or Sickledex reagent’ and the apparent solubilizing effect of urea on reduced hemoglobin S (3). The color of paired p...
متن کاملNonredox 5-lipoxygenase inhibitors require glutathione peroxidase for efficient inhibition of 5-lipoxygenase activity.
Nonredox type 5-lipoxygenase (5-LO) inhibitors, such as ZM 230487, its methyl analogue ZD 2138, or the Merck compound L-739,010, suppress cellular leukotriene synthesis of ionophore stimulated granulocytes with IC50 values of about 50 nM. However, in cell homogenates or in preparations of purified enzyme, up to 150-fold higher concentrations are required for similar inhibition of 5-LO activity....
متن کاملRapid spot test for the determination of esculin hydrolysis.
Esculin hydrolysis is a useful test in the differentiation of both gram-positive and gram-negative bacteria covering a wide spectrum of aerobes, facultative anaerobes, and anaerobes. Commonly utilized methods require a minimum of 18 h of incubation in broth or agar medium and utilize the production of a brown-black compound, due to the combination of ferric ions with the hydrolysis product escu...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1972
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)39425-6