Spontaneous oxygen radical generation by sickle erythrocytes.
نویسندگان
چکیده
منابع مشابه
Spontaneous oxygen radical generation by sickle erythrocytes.
Since the various membrane abnormalities of sickle erythrocytes might result from excessive accumulation of oxidant damage, we have measured the generation of superoxide, peroxide, and hydroxyl radical by normal and sickle erythrocytes using assays involving reduction of cytochrome c, aminotriazole inhibition of catalase, and methane evolution from dimethyl sulfoxide, respectively. Compared wit...
متن کاملOxygen-based free radical generation by ferrous ions and deferoxamine.
Deferoxamine accelerates the autooxidation of iron as measured by the rapid disappearance of Fe2+, the associated appearance of Fe3+, and the uptake of oxygen. Protons are released in the reaction. The formation of H2O2 was detected by the horseradish peroxidase-catalyzed oxidation of scopoletin, and the formation of hydroxyl radicals (OH.) was suggested by the formation of the OH. spin trap ad...
متن کاملOxygen radical generation by isolated microsomes from soybean seedlings.
The generation of active oxygen species by microsomes isolated from soybean seedlings was studied. NADPH-dependent superoxide anion production was 5.0 +/- 0.4 nmol . min(-1) mg(-1) of microsomal protein. Hydrogen peroxide generation by microsomes was 1.40 +/- 0.05 nmol . min(-1) mg(-1) of protein. Hydroxyl radical production, in the presence of ferric EDTA, evaluated through the generation of f...
متن کاملDenatured hemoglobin in sickle erythrocytes.
To study the nature of numerous inclusion bodies seen in red cells from patients with sickle cell disease (Hb SS), we have prepared red cell ghosts free of oxyhemoglobin and analyzed them by spectrophotometric and heme extraction methods. The absorption spectrum in the visible region of the ghost suspensions was typical of hemichromes. The spectrum was similar to that of denatured hemoglobin re...
متن کاملErythrocytes in Sickle Cell Anemia
patients, we have studied the viscosimetric and hemodynamic characteristics of four major classes of hemoglobin SS erythrocytes. We have isolated reticulocytes, discocytes, dense discocytes, and irreversibly sickled cells (fractions I-IV) on Percoll-Renografin density gradients. Bulk viscosity was studied in a coneplate viscosimeter and the hemodynamic studies were performed on the isolated, ar...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1982
ISSN: 0021-9738
DOI: 10.1172/jci110724