Spontaneous luteinization of antral marmoset follicles in vitro
نویسندگان
چکیده
منابع مشابه
The in vitro effect of chick embryo extract on mice pre-antral follicles
Chick embryo extract (CEE) contains a variety of growth factors which may improve in vitro follicle growth. Therefore, the effect of CEE on mouse pre-antral follicle culture was evaluated. Different percentages of CEE (0, 0.50%, 1.00%, 5.00% and 10.00%) were added to culture medium. Hence, the osmolarity of media was measured. Pre-antral follicles with diameter of 120-150 μm were isola...
متن کاملMethods of separation and in-vitro culture of pre-antral follicles from mammalian ovaries.
A variety of procedures to isolate intact pre-antral follicles have been developed in our laboratories based on different concentrations of collagenase and DNase in a simple salt solution containing glucose as an energy substrate. The enzyme mixture effectively separates follicles of different sizes derived from ovaries of hamster, mouse, rat, pig and human. While no damage of the basal lamina ...
متن کاملQuantitative bioluminescence imaging of transgene expression in intact porcine antral follicles in vitro
BACKGROUND The porcine oocyte maturation in vivo occurs within the ovarian follicle and is regulated by the interactions between oocytes and surrounding follicular components, including theca, granulosa, and cumulus cells, and follicular fluid. Therefore, the antral follicle is an essential microenvironment for efficient oocyte maturation and its developmental competence. Quantitative biolumine...
متن کاملIn Vitro Growth and Maturation of Vitrified-Warmed Bovine Oocytes Collected from Early Antral Follicles
Cryopreservation of growing oocytes enriches the choice of timing and location of artificial embryo production. However, completion of oocyte growth after warming is crucial when using such cryopreserved oocytes. Our research objective was to develop a sequential system that incorporates cryopreservation of growing bovine oocytes and their subsequent in vitro growth. Oocyte-granulosa cell compl...
متن کاملCapillary angiogenesis and degeneration in bovine ovarian antral follicles.
Angiogenesis and capillary degeneration are both evident during ovarian follicle growth. However, the characteristics and distribution of thecal capillary proliferative and degenerative structures have not been fully defined. Indeed, the role of thecal microvasculature changes in follicular atresia is still a matter of debate. The present study examined the distribution of thecal capillary chan...
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ژورنال
عنوان ژورنال: Molecular Human Reproduction
سال: 2000
ISSN: 1460-2407
DOI: 10.1093/molehr/6.6.504