Spontaneous excision of BAC vector sequences from bacmid-derived baculovirus expression vectors upon passage in insect cells
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چکیده
منابع مشابه
Spontaneous excision of BAC vector sequences from bacmid-derived baculovirus expression vectors upon passage in insect cells.
Repeated baculovirus infections in cultured insect cells lead to the generation of defective interfering viruses (DIs), which accumulate at the expense of the intact helper virus and compromise heterologous protein expression. In particular, Autographa californica multicapsid nucleopolyhedovirus (AcMNPV) DIs are enriched in an origin of viral DNA replication (ori) not associated with the homolo...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 2003
ISSN: 0022-1317,1465-2099
DOI: 10.1099/vir.0.19438-0