Spin-Label Studies of Rhodopsin-Lipid Interactions
نویسندگان
چکیده
منابع مشابه
Spin label saturation transfer ESR studies of protein-lipid interactions in Photosystem II-enriched membranes
Saturation transfer ESR has been used to study the dynamic behaviour of iipids in the appressed regions of thylakoid membranes from pea seedlings. Four different phospnoand galacto-lipid spin labels (phosphatidylcholine labelled ~t the 12 or 14 C-atom positions of the m-2 chain, phosphatidylglycerol labelled .~t the 14-1msition nf !he sn2 chaiii, and monogalaetosyldiacylglycerol labelled at the...
متن کاملSpin-label ESR studies of lipid-protein interactions in thylakoid membranes.
Lipid-protein interactions in thylakoid membranes, and in the subthylakoid membrane fractions containing either photosystem 1 or photosystem 2, have been studied by using spin-labeled analogues of the thylakoid membrane lipid components, monogalactosyldiacylglycerol, phosphatidylglycerol, and phosphatidylcholine. The electron spin resonance spectra of the spin-labeled lipids all consist of two ...
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The interactions of lysine oligopeptides with dimyristoyl phosphatidylglycerol (DMPG) bilayer membranes were studied using spin-labeled lipids and electron spin resonance spectroscopy. Tetralysine and pentalysine were chosen as models for the basic amino acid clusters found in a variety of cytoplasmic membrane-associating proteins, and polylysine was chosen as representative of highly basic per...
متن کاملLipid-protein interactions in stacked and destacked thylakoid membranes and the influence of phosphorylation and illumination. Spin label ESR studies.
The effects of membrane destacking, protein phosphorylation, and continuous illumination have been studied in pea thylakoid membranes using ESR spectroscopy of an incorporated spin-labelled phosphatidylglycerol. This spin-labelled analogue of an endogenous thylakoid lipid has previously been shown to exhibit a selectivity of interaction with thylakoid proteins. Neither destacking, phosphorylati...
متن کاملESR studies of spin-labeled membranes aligned by isopotential spin-dry ultracentrifugation: lipid-protein interactions.
Electron spin resonance (ESR) studies have been performed on spin-labeled model membranes aligned using the isopotential spin-dry ultracentrifugation (ISDU) method of Clark and Rothschild. This method relies on sedimentation of the membrane fragments onto a gravitational isopotential surface with simultaneous evaporation of the solvent in a vacuum ultracentrifuge to promote alignment. The degre...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1982
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(82)84617-1