منابع مشابه
Spectral imaging fluorescence microscopy.
The spectral resolution of fluorescence microscope images in living cells is achieved by using a confocal laser scanning microscope equipped with grating optics. This capability of temporal and spectral resolution is especially useful for detecting spectral changes of a fluorescent dye; for example, those associated with fluorescence resonance energy transfer (FRET). Using the spectral imaging ...
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We present a fiber-optic low-coherence imaging technique, termed spectral-domain differential interference contrast microscopy (SD-DIC), for quantitative DIC imaging of both reflective surfaces and transparent biological specimens. SD-DIC combines the common-path nature of a Nomarski DIC interferometer with the high sensitivity of spectral-domain low-coherence interferometry to obtain high-reso...
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Nuclear magnetic resonance ( N M R ) imaging is increasingly being used for medical diagnosis and in scientific research and applications. In practice, it is often difficult to quantitatively relate the image intensity to the nuclear spin density, because other factors complicate a proportional interpretation. These factors include chemical shift, magnetic susceptibility variation, nuclear spin...
متن کاملSpectral-domain optical coherence phase microscopy for quantitative phase-contrast imaging.
We describe a novel microscopy technique for quantitative phase-contrast imaging of a transparent specimen. The technique is based on depth-resolved phase information provided by common path spectral-domain optical coherence tomography and can measure minute phase variations caused by changes in refractive index and thickness inside the specimen. We demonstrate subnanometer level path-length se...
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The Internet is enabling greater access to spectral imaging publications, spectral graphs, and data than that was available a generation ago. The spectral imaging systems discussed in this issue of Cytometry work because reagent and hardware spectra are reproducible, reusable, and provide input to spectral unmixing and spectral components recognition algorithms. These spectra need to be readily...
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ژورنال
عنوان ژورنال: Optics Letters
سال: 2011
ISSN: 0146-9592,1539-4794
DOI: 10.1364/ol.36.003323