Specificity of Ribonucleic Acid Chain Initiation by Bacteriophage T3-induced Ribonucleic Acid Polymerase
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چکیده
منابع مشابه
Fidelity of in vitro transcription of T3 deoxyribonucleic acid by bacteriophage T3-induced ribonucleic acid polymerase and by Escherichia coli ribonucleic acid polymerase.
The fidelity of in vitro transcription of bacteriophage T3 DNA by T3 RNA polymerase and Escherichia coZi RNA POlymerase have been studied by DNA-RNA hybridizationcompetition studies with regard to (a) asymmetry of transcription and (b) the region of the T3 DNA transcribed by either polymerase. RNA transcribed by T3 RNA polymerase hybridized exclusively with the H strand of T3 DNA-the only stran...
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The kinetics of the RNA chain initiation reaction carried out by RNA polymerase bound to the initiator region of a DNA template have been analyzed. Initiation proceeds in a two-substrate reaction in which the initial binary complex (enzyme-DNA) is transformed into a ternary complex (enzyme-DNA-RNA) by formation of a dinucleoside tetraphosphate and release of inorganic pyrophosphate. In this rea...
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Recently attempts have been made to d&m: chemically the templates used as substrates in bacterial ribonucleic acid polymerase systems and to analyze the RX.1 products of such systems (l-9). Enzymatically synthesized polydeosynucleotides (2, 3, 6, 8) and polyribonucleotides (5, 7, 9, 10) permitted studies of the mechanism involved in the polyribonucleotide synthesis. It has been shown that an RX...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1974
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(20)79893-5