Specific Modification of Protein Sulfhydryl Groups with α,β-Unsaturated Compounds
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چکیده
منابع مشابه
Interaction of naphthomycin A with sulfhydryl compounds.
Naphthomycin A (1) was prepared as described previously1). I: C40H46CINO9; FD-MS m/z 719 (M+); shows UV 2MeOHmax"a°" n (e) 231 (38,900) and 275 (33,400). The elemental analysis revealed that I contains 4.49 % CI but no S. The retention time of I was 4.20 minutes by HPLC. Methanethiol, ethanethiol and propanethiol were purchased from Nakarai Chemical Co., Tokyo. p-Bromothiophenol was a product o...
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Egg albumin in the native, unaltered state, does not give tests characteristic of sulfhydryl groups. When, however, this protein is treated in any one of several ways, such as by heat (8, 9, 17), ultraviolet irradiation (lo), shaking (lo), or by solution in urea or other amides (7), free sulfhydryl groups make their appearance. The amount of free -SH groups appearing in egg albumin through the ...
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Bacillus subtilis glutamine synthetase readily reacts with iodoacetamide, producing very specific changes in the catalytic parameters characteristic of the native enzyme. The alkylation reaction is influenced both by substrates and feedback inhibitors of the enzyme; whereas ammonia and glutamate are without effect, Mg2+-ATP enhances the alkylation reaction, and Mn2f-ATP effectively prevents the...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1968
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)93315-6