Specific binding of oligoribonucleotide fractions toE. coliRNA polymerase

SPECIFIC AMPLIFICATION OF ASPERGILLUS FUMlGATUS DNA BY POLYMERASE CHAIN REACTION

Invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. An early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. Recently, the polymerase chain reaction (PCR) has been used successfully in detecting specific DNA of several pathogen. In this study, nested PCR was used to detect DNA specific for A!.pergiflus s...

An oligoribonucleotide polymerase from SV40-infected cells with properties of a primase.

A transient decaribonucleotide (iRNA) is covalently linked to nascent eukaryotic DNA chains at their 5' end. Searching for the putative iRNA polymerase (primase), we detected in extracts from SV40-infected cells a DNA-dependent incorporation of UMP residues from UTP into free and DNA linked deca- or similarly sized ribonucleotides. Denatured salmon sperm DNA served as the standard template in t...

The binding of a specific muscarinic antagonist to subcellular fractions of the bovine caudate nucleus.

adjuvant; a second intramuscular injection of an equal amount of antigen in Freund’s incomplete adjuvant was given 1 month later. Then 1 week later rabbits were bled, and a crude immunoglobulin preparation was made by the method of Kekwick (1940). The results shown in Fig. 1 suggest that the human neuroblastoma line contains monoamine oxidase activity of an A type, whereas that of MC63 cells is...

Application of specific primers and polymerase chain reaction for identification of ralstonia solanacearum biovar2

Sequence-specific cleavage of oligoribonucleotide capable of forming a stem and loop structure.

The precursor of an RNA molecule from T4-infected Escherichia coli cells (p2Sp1 RNA) has the capacity to cleave itself at specific positions ((UpA (137-138) and CpA (170-171)) within a possible loop and stem structure. This specific cleavage requires at least a monovalent cation and nonionic detergents. To confirm that the sequence-specific cleavage occurs autolytically, we studied the selectiv...