Species Checklistand DNA Barcoding of Baung (Bagrid Catfish) Hemibagrus Hoevenii from Muar River, Johor

Of Asia, Africa, and the Middle East, there are 15 genera in Bagridae family. The tropical freshwater catfish Hemibagrus hoeveniiis found Asian waters. Bagrids also known as Old World pimelodids, while New bagrids may be more accurate. In Muar, Johor, DNA barcoding has never been utilised to determine species of bagrid catfish. Therefore,this study was done updatethe checklist Baung (bagrid catfish)in Muar River,and protein-coding cytochrome coxidase I (COI) mitochondrial gene donefor identification phylogenetic analyses. A number two partial COI sequencesranging 674-687 nucleotide baseswere successfully obtained for specimens Lawi Nucleotide Basic Local Alignment Search Tool (BLAST) analysis suggested theirspecies status from genus Hemibagrus. Furthermore, results analyses showedthat neighbour joining tree, maximum parsimony tree likelihood grouped mtDNA sequencesof Lawifrom MuarRiverin one single cluster, thusconfirming showed presence H. hoeveniiin MuarRiver, Johor.