Spatial-temporal lipidomics profile of acute myocardial injury

Abstract Background Lipidome disturbance has long been recognized to occur after myocardial infarction (MI). Accumulation of excessive fatty acids induces production reactive oxygen species and consequently deteriorates cardiac injury in MI. However, the spatial temporal lipid profile heart following ischemic remains unknown. Purpose We aim uncover temporal-spatial lipidome ischemia reperfusion (I/R) identify circulating lipids released from injured myocardium that are potentially useful for diagnosis disease. Methods C57/BL6 mice were subjected 30 min followed by removal ligature establish injury. Porcine I/R was induced 105 reperfusion. Human plasma obtained 143 post-MI patients. Myocardial profiles generated matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MALDI-MSI) different regions (infarct, remote peri-infarct) at time points. Moreover, analysed LC-MS/MS. Results observed a drastic alteration with distinct spatial-temporal features both MALDI-MSI In infarct tissue, as revealed LC-MS/MS, we an elevation glycerolipids peaked 3 hours I/R, sustained phospholipids sphingolipids up days. Similar alternations but much weaker peri-infarct tissue compared tissue. Among those lipids, PC 32:0 detected highly overlapped CD68 staining single-cell level, showing strong correlation macrophage infiltration mouse hearts (R2=0.93, p<0.0001). A similar increase area also porcine Surprisingly, levels decreased humans, patients lower than healthy individuals (p=0.03). Further analysis demonstrated determined within 72 percutaneous coronary intervention negatively correlated 6-month ejection fraction (R2=0.08, p<0.001). Conclusions established synergizing LC-MS/MS imaging. positively function Our findings indicate is potential biomarker inflammatory status heart. Funding Acknowledgement Type funding sources: Public grant(s) – National budget only. Main source(s): Singapore Ministry Health's Medical Research Council