Spatial mapping of phosphorus influx in bean root systems using digital autoradiography

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Spatial mapping of phosphorus influx in bean root systems using digital autoradiography.

A novel technique was developed to spatially map the phosphorus net influx capacity in intact root systems. The method is based on digital autoradiography and permits the quantification of phosphorus influx at high spatial resolution (2 mm). Roots of 18-d-old common bean plants were exposed to (32)P-labelled orthophosphate, quickly frozen, excised, lyophilized, scanned, and exposed to a storage...

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ژورنال

عنوان ژورنال: Journal of Experimental Botany

سال: 2004

ISSN: 1460-2431

DOI: 10.1093/jxb/erh246