Southern Blotting of Long-Term Preserved DNA
نویسندگان
چکیده
منابع مشابه
Southern Blotting and Related DNA Detection Techniques
Southern blotting is one of the central techniques in molecular biology. First devised by E.M. Southern (1975), Southern blotting results in transfer of DNA molecules, usually restriction fragments, from an electrophoresis gel to a nitrocellulose or nylon sheet (referred to as a ‘membrane’), in such a way that the DNA banding pattern present in the gel is reproduced on the membrane. During tran...
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15 صفحه اولModified agarose gel for high-performance Southern blotting of short DNA fragments.
Southern blotting is a common method used for the study of gene organization. Current methods of DNA transfer for Southern blotting, however, can be inefficient for high concentration agarose gels. Here, we report a method for high-performance Southern blotting of short DNA fragments such as nucleosomal DNAs by using a discontinuous agarose zone gel. The results show that sharp and well-resolve...
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متن کاملDot and slot blotting of DNA.
Dot and slot blotting are simple techniques for immobilizing bulk unfractionated DNA on a nitrocellulose or nylon membrane. Hybridization analysis can then be carried out to determine the relative abundance of target sequences in the blotted DNA preparations. Dot and slot blots differ only in the geometry of the blot, a series of spots giving a hybridization pattern that is amenable to analysis...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1997
ISSN: 0736-6205,1940-9818
DOI: 10.2144/97224bm02