Somatic mutations and recombination test in Drosophila melanogaster used for investigating the genotoxicity of some food additives
نویسندگان
چکیده
In the present study, effects of several food colorings, namely (Ponceau 4R (E 124), Tartrazine 102), and Pea green 102-E 133), were investigated in vivo using wing spot test, SMART (somatic mutation recombination test), Drosophila melanogaster. Food colorings are additives, which used for improving appearance beverages. SMART, multiple hair (mwh), flare (flr3), beaded serrate (BdS) marker genes on third-largest chromosome used. The genotoxic imaginal disc cells that will develop into during embryonic development heterozygous larvae genotypic changes caused by or somatic also play a role formation mutant spots wings. Classes clones as follows: small single containing 1-2 mwh, large ?3 mwh ?4 flr3, twin adjacent flr3 (GRAF et al., 1984). Negative control medium was prepared with distilled water, while positive 1 mM EMS (ethyl methane sulfonate). According to results obtained from Ponceau 4R, Tartrazine, demonstrated significant trans-heterozygous flies (mwh/flr3) inducing compared groups at 25 mg/ml, 50 75 mg/ml exposure concentrations. On other hand, yielded balancer-heterozygous (mwh/TM3) numbers increased all three depending concentration (X2= df=3, P<0.001). It determined these significantly higher than negative it suggests have effects. However, less medium; this finding indicates not much EMS.
منابع مشابه
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ژورنال
عنوان ژورنال: International journal of agriculture, environment and food sciences
سال: 2021
ISSN: ['2602-246X', '2618-5946']
DOI: https://doi.org/10.31015/jaefs.2021.1.9