Solution-based circuits enable rapid and multiplexed pathogen detection

Rapid molecular techniques for detection of foodborne Bacillus cereus pathogen

Background and Objective: Bacillus cereus is responsible for several outbreaks of foodborne diseases. Therefore, the purpose of the study was to replace routine culture testing, by employing PCR directly from food to detect the bacteria and its enterotoxins. Methods: In the present study, a total of 75 Kibda sandwiches, Sausage sandwiches, Luncheon chicken, Luncheon meat and shawarma sandwiche...

Nanoparticle-based DNA multiplexed probes for pathogen detection using confocal raman microscopy

Rapid Waterborne Pathogen Detection with Mobile Electronics

Pathogen detection in water samples, without complex and time consuming procedures such as fluorescent-labeling or culture-based incubation, is essential to public safety. We propose an immunoagglutination-based protocol together with the microfluidic device to quantify pathogen levels directly from water samples. Utilizing ubiquitous complementary metal-oxide-semiconductor (CMOS) imagers from ...

Radioluminescent nanophosphors enable multiplexed small-animal imaging

We demonstrate the ability to image multiple nanoparticle-based contrast agents simultaneously using a nanophosphor platform excited by either radiopharmaceutical or X-ray irradiation. These radioluminescent nanoparticles emit optical light at unique wavelengths depending on their lanthanide dopant, enabling multiplexed imaging. This study demonstrates the separation of two distinct nanophospho...

Rapid Isothermal Amplification and Multiplexed Surface-Based Detection of Short DNA Sequences

We have coupled a novel isothermal amplification method for short oligonucleotides (EXPAR) with solution and surface-based detection involving DNA-functionalized gold nanospheres. The assay allows for different target sequences to be detected using the same set of DNA:Au nanospheres through appropriately designed reporter sequences. The solution based assay relies on simple visual detection thr...