Solid‐Phase Lipase‐CuNPs Biohybrids as Catalysts for One‐Pot Parallel Synthesis of 2,3,4‐Triacetyl‐D‐Gluconic Acid

Solid-phase lipase/metal nanobiohybrids, generated by growth of copper nanoparticles on enzyme matrixes immobilized graphene, were used as heterogeneous catalysts with dual-activity for the regioselective production 2,3,4-triacetyl-D-gluconic acid from α-peracetylated-glucose in a one-pot parallel process combining lipase-mediated hydrolytic monodeprotection metal-catalyzed oxidation aqueous media. A novel synthetic strategy, based situ fabrication Cu induced lipase molecules specifically multi-layer graphene material interfacial adsorption fixing them active open conformation, has been described. Thermomyces lanuginosus was firstly to prepare functionalized graphite biographene preparation (Biographene, BIOG), support successfully immobilize Candida rugosa (CRL). This form BIOG-CRL further create successful bifunctional enzyme-metal nanoarchitectures. Two different Cu-lipase hybrids synthesised, where species and size depending methodology. Regioselectivity stability evaluated monosaccharide building blocks, besides robustness recyclability experiments. These findings highlight potential these solid-phase nanoarchitectures useful tools synthesis complex glycoderivatives use food, medicine, cosmetics.