Solid/liquid separation by sedimentation

Sedimentation for Analysis and Separation of Macromolecules

A ‘centrifugal force’ as experienced by particles in a centrifuge actually reflects the natural tendency of the particle to move in a straight line in the absence of external forces. If we view the experiment in the (fixed) reference frame of the laboratory, we observe that the particle is deflected from a linear trajectory by a centripetal force (directed toward the central axis of the rotor)....

Separation of rabbit adrenal cells by sedimentation at a linear gradient of albumin concentration.

The isolated cells of rabbit adrenal glands were separated by the method of sedimentation at a linear gradient of albumin concentration and incubated with 14C-cholesterol. The corticosterone and cortisol produced by the separated cells were fractionated by thin-layer chromatography and the radioactivities in these steroids were measured. The more rapidly sedimenting cells showed a greater ratio...

Separation of erythroid progenitor cells in mouse bone marrow by isokinetic-gradient sedimentation.

Isokinetic-gradient sedimentation employing a shallow linear gradient of Ficoll in tissue culture medium was used to isolate erythroid progenitor cells (CFU-e) from mouse bone marrow. Following gradient sedimentation, 34% of the total nucleated cells and 48% of the CFU-e applied to the gradient were recovered, and three distinct modal populations of CFU-e could be distinguished. The slowest-mig...

Isolation and separation of highly enriched fractions of viable mouse gastric parietal cells by velocity sedimentation

Methods of tissue dissociation and cell separation have been modified to obtain highly enriched fractions of mouse gastric parietal cells. Suspension of gastric mucosal cells are prepared by pronase digestion of the glandular portion of the stomach from adult mice. By utilizing the velocity sedimentation technique to separate cells of different sizes it is possible to recovery parietal cells, w...

Separation of immunocompetent cells from human and mouse hemopoietic cell suspensions by velocity sedimentation.